Effects of Qingkailing Injection on Expression of Tight Junction Claudin 5 in the Hypoxia Model of Brain Microvascular Endothelial Cells
Abstract：M edicine o fM inistry o f Educa tion and Beijing, Dongzhim en Hospita l,Be ijing University o f Chinese M edicine(Be ijing 100700), China ABSTRACT :O bjective To observe the effects o f hypoxia on expression o f tight junction claudin 5 o f bra in m icrovascular en-do the lia l ce ls and the ro le o fQ ingka iling injection.M e thods M ouse cerebra lm icrovascula ture endo the lia l ce ls o f Ba lb/c w as cul-tured and divided into five g roups :Contro lg roup,m ode lg roup, Q ingka iling low, m edium and high concentra tion g roups. The m ode lo f ischem ic injury w as established by oxygen and sugar deprived fo r 24 h.Rea l T im e PCR w as used to eva lua te the gene expression o f claudin 5,and pro te in expression w as tested by w estern blo t.Results C om pared w ith contro l g roup,the expression o f claudin5 w as reduced significantly after oxygen and sugar be deprived fo r 24 h( P < 0.05). Com pared w ith the m ode l g roup, gene expres-sion in the low and high dose o fQ ingka iling injection increased significantly and claudin 5 pro te in im proved obviously in the m edium g roup.Conclusion Q ingka iling injection had cerebra lpro tection ro le in ischem ic stroke by recovering the barrier function through in-creasing the expression o f claudin 5.
Keywords：brainmicrovascularendothelialcelQingkailing Injectionhypoxiaclaudin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1363-1365 )