Effects o fMDQR4 on Acute M yocardia l Ischem ia Reperfusion Injury in Ra ts

Fu Xiaochun; Huang haichao; Luo Yanna

doi:10.3969/j.issn.16721349.2014.11.028

Abstract：O bjective To study the effect o fMDQR4 on acute m yocardia l ischem ia reperfusion( I/R) in rats. Methods M anufac-ture the m ode l o fm yocardia l I/R in rats. U sing kit to observe the leve ls o f serum crea tine kinase(CK) ,tum o r necrosis facto r a lpha (TNF α) and interleukin 1β( IL 1β). and the infarct size o f each group w ere m easured by TTC sta ining. And the leve l o f NF κB w as a lso de tected.And contento fm a londia ldehyde(MDA) and superoxide dism utase(SOD) in m yocardia l tissue w ere observed. Re-sults Fo low ing ischem ia 20m in and reperfusion 40m in,serum concentra tion o f TNF α ,IL 1β and CK in I/R group and m edicine pre trea tm ent group w as significantly increased com pared w ith tha t in sham group,and serum concentra tion o fTNF α ,IL 1β and CK in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group. Fo low ing ischem ia 20 m in and reperfusion 40 m in,infarct size and the expression o fNF κB in I/R group and m edicine pre trea tm ent group w as significantly increased. And infarct size and the expression o f NF κB in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group. Fo low ing reperfusion 40m in ,MDA contentw as significantly increased and SOD contentw as significantly reduced com pared w ith tha t in sham group,and MDA content in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group ,and SOD content in ev-ery m edicine pre trea tm ent group w as significantly higher than tha t in I/R group. Conclusion MDQR4 could reduce the infarct size and inhibitNF κB activa tion and dow n regula te the expression o f TNF α ,IL 1β ,CK ,MDA and increase the content o f SOD ,w hich m ay be one o f the m echanism s o f its cardiopro tection.