Bax Gene Expression After Hypoxic ischemic Brain Injury by Mild Hypothemia Intervention in Neonatal Rats
Abstract：Objective To observe the changes of Bax gene expression in brain tissue after hypoxia ischemia brain damage (HIBD),and explore the possible protection mechanism of mild hypothermia.Methods A total of 88 seven day old neonatal rat models of HIBD established by Rice method were randomly divided into three groups:Sham operated group,HIBD group,and mild hypothermia intervention group.The experimental group were further divided into five sub groups (n=8 each)based on different time points after HIBD (2 h,6 h,12 h,24 h,48 h),respectively.Fluorescence quantitative PCR (PT PCR)technique was used to de-termine the expression changes of Bax mRNA in brain tissues.Results There was no significant change in Bax mRNA expression in control group.The expression was increased at 2h after HIBD,12h to the peak level,12 48h gradually increased (P<0.05).Bax mRNA expression in mild hypothermia intervention group was significantly lower than that of HIBD group at each time points (P<0.05).Conclusion Bax mRNA participated in HIBD of neonatal rats.Mild hypothermia intervention could reduce the expression of Bax mRNA in brain tissue after HIBD.Therefore,hypothermia intervention had some neuroprotective effects on the brain tissues of neonatal rats of HIBD.
Keywords：hypoxia ischemia damagebrainhypothermiaBaxrat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1114-1116 )