Anti atherosclerotic Effect of Bear Bile Powder in Shexiang Tongxin Dripping Pills on ApoE/Mouse

doi:10.3969/j.issn.16721349.2014.09.039

Abstract：Objective To study the anti atherosclerotic activity of bear bile powder (BBP)in Shexiang Tongxin dripping pills (STDP)on ApoE / mice.Methods ApoE / mice fed with high fat diet were utilized to reproduce animal models of atheroscle-rosis.ApoE / mice were treated with bear bile powder aqueous solution,STDP solution and STDP depleted with bear bile power so-lution,respectively.Interventional effect on the atherosclerotic lesion size of aorta and aortic root were measured by hematoxylin&eosin staining,oil red O staining and masson’s trichrome staining,respectively.Serum levels of total cholesterol (CHO),triglyceride (TG), high density lipoprotein (HDL)and low density lipoprotein (LDL)were determined by biochemical method.Results Compared to that from the ApoE / mice treated with vehicle,three treated groups were marked with a significant decrease in the size and extent of atherosclerotic lesions(P<0.05).As expected,a better therapeutic effect was observed in STDP treated mice compared to that from the mice receiving STDP depleted with bear bile power.Compared to that from the ApoE / mice treated with vehicle,in the serum levels of CHO and TG levels was decreased(P<0.01),increased serum HDL (P<0.01)was also noted in three groups of mice receiving either BBP,STDP or STDP without BBP.Furthermore,lower level of LDL was noted in BBP treated mice.Conclusion A BBP contributes significantly in mediating the anti atherosclerotic effects of STDP,in particular,in reducing serum LDL level in ApoE / mouse model.