Effect of Hesperetin on Vasorelaxation of Isolated Thoracic Aortic Rings of Rat
Abstract：Objective To investigate the effect of hesperetin (HSP)on the isolated thoracic aortic rings of rats and to explore under-lying the mechanism of the HSP.Methods The isolated thoracic aortic rings of male Sprague Dawley (SD)rats were mounted on the organ bath and tension was recorded.The effect of HSP on the isolated thoracic aortic rings with intact endothelium or denuded endo-thelium was precontracted by the potassium chloride (KCl,60 mmol/L)or phenylephrine (PE,10-6 mol/L),and the effects of HSP on the blood vessels reaction induced by various drugs were recorded with Powerlab biological signal assay system.Results HSP completely vasorelaxed the contractions induced by KCl and PE in a concentration dependent manner in endothelium intact and endo-thelium denuded rat aortic rings.When the contractions was induced by KCl and PE,L NAME (10-4 mol/L),Indo (10-5 mol/L)and BaCl2 (10-3 mol/L)had no significant inhibition on the vasorelaxant effects of HSP (P>0.05).When the contractions was induced by KCl,4 AP (10-3 mol/L)could weaken HSP diastolic function (P<0.05).When the contractions was induced by PE,4 AP (10-3 mol/L)could weaken HSP diastolic function (P<0.05).Conclusion HSP vasorelaxed the contractions induced by KCl and PE in a concentration dependent manner in endothelium intact and endothelium denuded rat thoracic aortic rings.Its mechanism might be related to the voltage dependent potassium channel (Kv).
Keywords：hesperetin thoracic aortic ringsvascular smooth musclepotassium channel
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 870-872 )