Effects of Nuclear Factor kappa B Expression in Hippocampal Nerve Cells of Epileptic Rats through Dazhen Acupuncture Manipulation on Du Meridian

doi:10.3969/j.issn.16721349.2014.04.045

Abstract：Objective To study the effects of nuclear factor kappa B(NF κB)expression in hippocampal nerve cells of epileptic rats through dazhen acupuncture manipulation on Du Meridian,then explore the mechanism of anti epilepsy through dazhen acupunc-ture manipulation on Du Meridian,provide new theoretical basis for clinical acupuncture treatment of epilepsy.Methods Thirty health rats were randomly and enenly divided into blank control group(group A),epilepsy model group(group B),dazhen acupuncture manipulation group(group C),Lithium chloride and pilocarpine were injected the rats’enterocoelias of group B and group C,induce status epilepticus(SE)model.The rats’behavior changes were observed.Equal dose of normal saline were injected the rats’entero-coelia of group A.Any treatment was not given to group A,dazhen acupuncture manipulation was applied at points of Du Meridian :Fengfu,Dazhui,Taodao,Wuming,Shenzhu,once a day,20 minutes every time,treatment for 14 days.The pathological morphological changes in the hippocampal nerve cells of the three groups were observed by using HE staining.The expression of NF κB P65 in hip-pocampal nerve cells of the epileptic rats were observed by immunohistochemistry.Results The status epilepticus were induced in group B and group C,one rat dead in two groups.The rats in group B had obvious pathological morphological changes,the pathologi-cal morphological change in group C had obvious improvement.There was no obvious pathological morphological changes in group A.Immunohistochemical staining:The expression of NF κB P65 was(13.95±6.99)in group A,(28.75±8.80)in group B,(19.25± 8.60)in group C.Comparing each two groups,the differences are statistically significant(P <0.05).Conclusion Dazhen acupuncture manipulation on Du meridian,maybe can suppress the expression of NF κB P65 in the hippocampal nerve cells of epileptic rats,then reduce the hippocampal nerve cells apoptosis,treat epilepsy.