Effect of Homocysteinemia on Proliferation of Human Umbilical Vein Endothelial Cell
Abstract：Objective To investigate proliferation and morphological structure of human umbilical vein endothelial cells (HUVEC) incubated with homocysteine.Methods Endothelial cells were incubated in 1640 cultivate medium with different concentrations of homocysteine and the control cultivate medium for 24 h,48 h,and 72 h.Then cell proliferation activity and cell morphology were detected by MTT colorimetric assay.Results The cell growth inhibition was not obvious in low concentrations of homocysteine.As the concentrations were increased,cell growth inhibition was more obvious dose-dependently and time-dependently.Conclusion Hyperhomocysteinemia could damage the endothelial cells,inhibit cell growth,and contribute to the occurrence and development of atherogenic vascular disease of diabetes and insulin resistance.
Keywords：human umbilical vein endothelial cellhyperhomocysteineamiadiabetes mellitusatherosclerosis
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 938-939 )