Exploration of the Mechanism of Ganmai Dazao Decoction in Treating Depression Based on Network Pharmacology and Machine Learning
LIANG Ke
ZHAO Shuai
GAO Yuguang
HE Qianchao
Abstract：Objective This study aims to investigate the potential molecular mechanisms of Ganmai Dazao Decoction(GMDZD)in treating depression based on network pharmacology and machine learning,providing a scientific basis for modern research on traditional Chinese medicine.Methods Active ingredients and their targets of GMDZD were screened using databases such as TCMSP( HERB( expressed genes related to depression were obtained from the GEO database( GSE98793,GSE19738,and GSE44593.Core genes were identified using Random Forest and LASSO regression methods,and a drug-component-target network was constructed.Key pathways were revealed through gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses.A transcription factor-miRNA regulatory network and immune infiltration analysis were used to explore its immunomodulatory mechanisms.Results A total of 117 active ingredients(e.g.,quercetin,stigmasterol)and 126 overlapping targets were identified.Core targets including ADORA3,APH1B,and PLAU(AUC＞0.5)were found to exert therapeutic effects by regulating pathways such as neuroactive ligand-receptor interaction and signal transduction.In terms of biological processes,the targets were mainly involved in response to external stimuli,hormone metabolic processes,and steroid metabolic processes.Cellular component analysis highlighted membrane rafts,membrane microdomains,and plasma membrane rafts.Molecular function analysis indicated involvement in G-protein coupled amine receptor activity and neurotransmitter receptor activity.Immune analysis revealed a significant positive correlation between APH1B and immune cells such as neutrophils and M0 macrophages.Conclusion GMDZD may ameliorate depression through multi-target mechanisms involving the regulation of monoamine neurotransmitters and the immune microenvironment.The interaction between its core components and targets provides a theoretical basis for subsequent experimental validation and drug development.
Keywords：Ganmai Dazao Decoctiondepressionnetwork pharmacologymachine learning
Publication Date：2026-05-20
Online Publishing Date：2026-07-17(First online date of this platform, not the publication date of the document)
Pages：9( 20-28 )