Mechanism of Kunling Pill in Improving Ovarian Function in DOR Rats Via the NF-κB/I-κB Pathway
WANG Haiyan
WEI Bowen
CAO Dan
Abstract：Objective To investigate the mechanism by which Kunling Pill improves cyclophosphamide(CTX)-induced diminished ovarian reserve(DOR)in rats by regulating the nuclear factor kappa B(NF-κB)/inhibitor of nuclear factor kappa B(I-κB)signaling pathway.Methods 45 Sprague Dawley(SD)female rats with normal estrous cycles were randomly divided into a normal control group,a model group,a positive drug group(estradiol valerate),a low-dose Kunling Pill group(0.5 g/kg),and a high-dose Kunling Pill group(1 g/kg),with 9 rats in each group.Except for the normal control group,all groups were established with a single injection of CTX(90 mg/kg)to establish a rat DOR model.After 28 days of continuous oral administration,serum hormones estradiol(E2),anti-Müllerian hormone(AMH),follicle stimulating hormone(FSH),inflammatory factors interleukin-1 beta(IL-1 β),interleukin-4(IL-4),interleukin-6(IL-6),and ovarian tissue NF-κB/IκB protein expression were detected.Results As a result,Kunling Pill can regulate the hormone levels in rats.Compared with the model group,the low-dose Kunling Pill group and high-dose Kunling Pill group showed significant increases in serum AMH and E2,while FSH showed no significant changes.Kunling Pill reduces the accumulation of IL-1β and IL-6 in the serum of DOR rats,and lowers the levels of NF-κB and I-κB to alleviate inflammatory reactions.Conclusion Kunling Pill can improve ovarian reserve function.The mechanism may be mediated by inhibiting the NF-κB/I-κ B pathway to alleviate inflammation induced ovarian damage and improve rat ovarian function.
Keywords：Kunling Pillovarian reserveNF-κB/I-κBinflammation
Publication Date：2026-01-20
Online Publishing Date：2026-07-17(First online date of this platform, not the publication date of the document)
Pages：6( 9-14 )