Study on the Effect of Kudzu Root Enzyme Complex on Improving Alcoholic Hepatitis
XUE Shuwen
ZHANG Guodong
RAO Zhiming
ZOU Xiaoji
YANG Ziyan
LIU Shimin
FU Dan
QIAN Kai
Abstract：Objective Based on the established optimal gavage dose,this study further investigated the pharmacological effects of the kudzu enzyme complex on alcoholic hepatitis in mice.Methods(1)Experimental grouping and administration:Fifty mice were randomly divided into a control group(Con),a model group(Mod),a low-dose kudzu enzyme complex group[JS(L)],a medium-dose kudzu enzyme complex group[JS(M)],and a high-dose kudzu enzyme complex group[JS(H)],with 10 mice per group.Dosing began on the fourth day of modeling:JS(L)15 mL/(kg·d),JS(M)30 mL/(kg·d),JS(H)60 mL/(kg·d),while the Con group received an equal volume of physiological saline,continuously for 14 days.After the experiment,mice were euthanized,and liver tissue and orbital venous plexus blood were collected.Detection indicators:calculation of liver index;observation of hematoxylin-eosin(HE)staining;Western blot detection of transforming growth factor-β(TGF-β),hypoxia-inducible factor-1α(HIF-1α),3-hydroxy-3-methylglutaryl-CoA reductase(HMG-CR),and sterol regulatory element-binding protein-1C(SREBP-1C)protein levels;measurement of alanine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),and total bilirubin(TBIL)using assay kits.(2)Experimental grouping and administration:Forty mice were randomly divided into the Con group,the kudzu enzyme complex group(JS),the Mod group,and the treatment group(Mod+JS),with 10 mice per group,using the same modeling method as in(1).After the experiment,mice were euthanized,and liver tissue and orbital venous plexus blood were collected.Detection indicators:observation of HE staining;Western blot detection of cyclooxygenase(COX),catalase(CAT),Bcl-2-associated X protein(BAX),B-cell lymphoma-2(BCL-2),caspase-3,caspase-9,interleukin-6(IL-6),interleukin-1(IL-1),tumor necrosis factor-α(TNF-α),TGF-β,HIF-1α,HMG-CR,SREBP-1C,phosphorylated calcium/calmodulin-dependent protein kinase 2(P-CaMK2),and phosphorylated AMP-activated protein kinase(P-AMPK)protein levels;measurement of ALT,AST,ALB,and TBIL using assay kits.Results(1)Compared with the Mod group,the JS(L),JS(M),and JS(H)groups showed concentration-dependent changes in liver index,fibrosis,and steatosis;HE staining results indicated concentration-dependent improvement in the JS(L),JS(M),and JS(H)groups compared to the Mod group.(2)Compared with the Mod group,the Mod+JS group showed significant improvements in liver indicators,fibrosis,and steatosis;HE staining results revealed disordered liver tissue structure,significant inflammatory cell infiltration,and lipid vacuoles in the Mod group compared to the Con group,while the Mod+JS group exhibited marked improvement compared to the Mod group;Western blot results demonstrated that the Mod+JS group effectively reduced the expression levels of inflammatory factors(IL-1,IL-6,TNF-α)and apoptosis-related factors(Caspase-9,Caspase-3,TGF-β)while enhancing antioxidant levels(COX,CAT)in the livers of mice with alcoholic hepatitis;further detection showed significantly increased expression of P-AMPK and P-CaMK2 in the Mod+JS group compared to the Mod group.Conclusion The kudzu enzyme complex can ameliorate alcoholic hepatitis in mice by modulating the expression of oxidative stress markers,inflammatory factors,and apoptosis-related factors in the liver.
Keywords：pueraria root enzyme complexalcoholic hepatitisoxidative stresscell apoptosisinflammatory factors
Publication Date：2025-11-20
Online Publishing Date：2026-07-17(First online date of this platform, not the publication date of the document)
Pages：8( 9-16 )