Study on Mechanism of Regulating Autophagy,Apoptosis and Scorch Death of Asthmatic Cells by Yishen Chuanning Decoction
LIU Ying
WANG Tongtong
DONG Yu'ang
Abstract：Objective Study on the regulation of lipopolysaccharide(LPS)-induced autophagy and related mechanisms in human bronchial epithelial cells(16HBE)by Yishen Chuanning Decoction.Methods The experiment was divided into 5 groups:normal group,model group,traditional Chinese medicine group,dexamethasone group,and traditional Chinese medicine+dexamethasone group.Cell counting kit-8 assay(CCK-8)was used to detect cell proliferation activity and screen for the optimal dose of Yishen Chuanning decoction;Observation of cellular damage using lactate dehydrogenase(LDH)release assay;Observation of changes in autophagosomes by transmission electron microscopy;Autophagy double labeled adenovirus experiment was used to detect changes in autophagic flux;UNEL staining was used to observe the apoptosis rate of cells;Immunofluorescence detection of cysteine dependent aspartate specific protease-1(Caspase-1)expression;Western blot was used to detect the protein expression of interleukin-1 β(IL-1β),interleukin-18(IL-18),Caspase-1,caspase-3,Beclin-1,and autophagy related protein 5(Atg-5).Results The CCK-8 experiment showed that the concentration of Yishen Chuanning decoction freeze-dried powder was 20%,and the best effect was achieved after 48 hours of cultivation.Observation by transmission electron microscopy showed that the normal group had normal cell morphology,intact cell membrane,clear mitochondrial cristae structure,and more autophagosomes;Model group,with normal mitochondrial morphology and fewer autophagosomes;The traditional Chinese medicine group showed mitochondrial shrinkage,reduced cristae,increased membrane density,and an increase in autophagosomes compared to the model group;The dexamethasone group showed mitochondrial swelling,reduced cristae,and a similar number of autophagosomes as the traditional Chinese medicine group;The group treated with traditional Chinese medicine and dexamethasone showed mitochondrial swelling,reduced cristae,and a decrease in the number of autophagosomes compared to the group treated with traditional Chinese medicine.The autophagy double labeled adenovirus experiment showed that the model group had significantly fewer yellow spots,autophagosomes,and lysosomes than the other groups,and the yellow spots were fewer than the red spots,indicating lower autophagy activity;The red and yellow spots in the traditional Chinese medicine group were reduced compared to the normal group and the traditional Chinese medicine+dexamethasone group,indicating a decrease in autophagosomes and autophagosomes.LDH and inflammatory factor detection showed that compared with the model group,the LDH leakage rate,IL-18,IL-1β,and Caspase-1 were significantly reduced in the normal group,traditional Chinese medicine group,dexamethasone group,and traditional Chinese medicine+dexamethasone group(P<0.01);The apoptosis rate,Atg-5,Beclin-1,and Caspase-3 significantly increased(P<0.01);Compared with the traditional Chinese medicine group,the LDH leakage rate in the normal group and the group treated with traditional Chinese medicine and dexamethasone was significantly reduced(P<0.01),while the apoptosis rate,Atg-5,Beclin-1,and Caspase-3 were significantly increased(P<0.01).Conclusion Yishen Chuanning Decoction can inhibit the survival rate of human bronchial airway epithelial cells,reduce cell autophagy and pyroptosis,and promote cell apoptosis.
Keywords：Yishen Chuanning Decoctionepithelioid cells in the bronchial airwayautophagypyroptosisapoptosis
Publication Date：2025-11-20
Online Publishing Date：2026-07-17(First online date of this platform, not the publication date of the document)
Pages：8( 1-8 )