Effect of MCC950 Regulation of Mitochondrial Pyruvate Carrier on Tau Protein in Alzheimer's Disease Model
QI Yue
AN Yang
DONG Xiaobo
XIA Chunpeng
WANG Jixian
FAN Guangkun
LI Jitong
LI Zhao
Abstract：Objective To investigate the mechanism of MCC950 reducing Tau protein in Alzheimer's disease model by inhibiting mitochondrial pyruvate carrier(MPC).Methods SH-SY5Y cells were induced by gonadic acid(20 nmol/L),supplemented with UK5099(1 μmol/L),and the survival rate of SH-SY5Y cells was detected by CCK8 method.The expressions of AT-8 was determined by immunofluorescence method.Active oxygen species was determined by DCFH-DA method.Mitochondrial membrane potential was measured by Rho 123.The expression levels of MPC and IL-1β were determined by Western blot.The levels of NLRP3,IL-1β and IL-18 were determined by ELISA.Results MCC950 had a protective effect on SH-SY5Y cells at 10 μmol/L concentration(P<0.01).It could increase the mitochondrial membrane potential,decrease the production of reactive oxygen species,and inhibit the expression of AT-8,Tau-5,IL-18,IL-1β and MPC(P<0.05 or P<0.01),but it had no signicant inhibitory effect on NLRP3.Conclusion MCC950 can reduce the expression of inflammasome and inhibit Tau hyperphosphorylation in okada-induced SH-SY5Y cells.The mechanism is related to inhibiting mitochondrial pyruvate carrier and improving mitochondrial function.
Keywords：MCC950Alzheimer's diseasemitochondrial pyruvate carrierTau proteinmitochondrial membrane potentialoxidative stress
Publication Date：2024-11-20
Online Publishing Date：2026-07-17(First online date of this platform, not the publication date of the document)
Pages：7( 6-12 )