Comparative Study of the Advantages of Fluorescence Quantitative PCR in Diagnosing Pulmonary Tuberculosis
LI Fuqiong
FENG Chengqi
WEI Qin
YANG Tongsen
Abstract：Objective:To analyze the diagnostic value of polymerase chain reaction (PCR),mycobacterium tuberculosis culture in bronchoscope lavage fluid and brush antacid detection (brush method) in the clinical diagnosis of pulmonary tuberculosis. Method:160 patients admitted to Qingzhen Hospital of Traditional Chinese Medicine from January 2022 to December 2023 were selected as research objects and divided into pulmonary tuberculosis group and non-pulmonary tuberculosis group according to whether they had tuberculosis,with 80 cases in each group. A total of 160 samples of alveolar lavage fluid were sent for examination. PCR,culture method and brush method were used to diagnose and analyze the standard of alveolar lavage fluid,and the diagnostic efficiency of the three kinds of detection methods was compared. With the results of Mycobacterium tuberculosis culture in bronchoscopic alveolar lavage fluid as the gold standard,the Kappa values detected by PCR and brush method were analyzed and calculated,and the positive predictive value (PPV) and negative predictive value (NPV) under the three detection methods were compared. Result:The postive rate of PCR diagnosis in pulmonary tuberculosis group was higher than that of culture method and the difference was not statistically significant( x2=16.667,P<0.05). The postive rate of culture method was higher than that of brush method,and the difference was statistically significant(x2=10.03,P<0.05)Among the three methods,PCR had the highest sensitivity and specificity,followed by culture method and brush method had the lowest. The consistency of PCR detection was good (Kappa=0.82),and the consistency of brush detection was general (Kappa=0.63). There was no significant difference in positive predictive value among the three diagnostic methods (P>0.05). The negative predictive value of PCR was higher than that of brush method,and the difference was statistically significant (P<0.05). Conclusion:Fluorescent quantitative PCR has a high diagnostic efficiency in the diagnosis of pulmonary tuberculosis,the diagnostic advantages are obvious,and can be used as a method.
Keywords：Bronchoalveolar lavage fluidPulmonary tuberculosisDiagnosisCulture methodPCRSmear method
Publication Date：2024-10-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 61-64 )