Characteristics of Intestinal Flora in Children with Autism Spectrum Disorder and Its Influence on Children's Behavior

HAN Junlin; LIN Yumei; LIN Kun; HAN Xinrong; WU Xingxing; ZHOU Shaojun; LIN Yizhen

doi:10.14033/j.cnki.cfmr.2023.34.016

Abstract：Objective:To analyze the characteristics of intestinal flora in children with autism spectrum disorder and its influence on their behavior.Method:Thirty children with autism spectrum disorder who admitted to Affiliated Hospital of Putian University Medical Group from June to September 2022 were included in the autism spectrum disorder group,and 20 healthy children matching age and sex were selected to be included in the control group during the same period.Fecal samples were collected,and the distribution of intestinal flora was determined by genomic pathogenic microorganism analysis and detection technology,and the autism-related pathogenic microorganisms were screened by information biology method.The intestinal flora richness index,the relative abundance of each flora and the autism treatment evaluation checklist(ATEC)score of two groups were compared,the correlation between intestinal flora characteristics and behavioral symptoms were analyzed.Result:There were no statistically significant differences in Sobs,Chao and abundance-based coverage estimator(Ace)between two groups(P>0.05);at the operational taxonomic units(OTU)level,the Shannon index and Shannoneven index of the autism spectrum disorder group were lower than those of the control group,and the differences were statistically significant(P<0.05).The relative abundance of Solobacterium,Prevotella and Bacillus in the autism spectrum disorder group were lower than those in the control group,and the relative abundance of Micrococcus,Comamonas,Burkholderia,Haldemania and Alistipes were higher than those in the control group,and the differences were statistically significant(P<0.05).The ATEC scores of the autism spectrum disorder group were higher than those of the control group,and the differences were statistically significant(P<0.05).The Shannon index,Shannoneven index and relative abundance of each bacteria in children with autism spectrum disorder were correlated with behavioral symptoms(P<0.001).Conclusion:The intestinal flora structure of children with autism spectrum disorder are closely related to their behavioral symptoms.