Application of Three Methods in Eliminating Interference of Chylemia in Analysis of Blood Cells

doi:10.14033/j.cnki.cfmr.2016.24.024

Abstract：Objective:To investigate the method for eliminating interference of chylemiaon on the main indexes of COULTER LH750 hematology analyzer. Method:Chyle blood samples which had been cleared by daily testing instruments was selected,they were detected and analyzed by one of the three methods ofdilution,blank,replacement randomly within 2 hours mainly to analyze the 13 parameters of RBC,WBC,PLT,Hb,HCT,MCV,MCH,MCHC,NEU%, LYM%,MON%,EOS%,BASO%,a total of 95 samples were detected.The content of triglyceride of all experimental remaining specimens were tested by blood biochemical analyzer,specimens with TG<6.0 mmol/L were selected as mild to moderate chylemia group,specimens with TG≥6.0 mmol/L were selected as severe chylemia group,the indexes of each method were compared before and after chyle processing.Each method in each experiment,at least one fresh specimen with fat free,no hemolysis,no jaundice was selected as control in order to assess the variation of each method.Result:The differences in the detection indexes of each control group of the three methods before and after processing were not statistically significant(P>0.05).The differences in Hb,MCH of chylous mild to moderate group before and after processing by dilution method were statistically significant(P<0.05).But in the chylous severe group,the Hb,MCH,MCHC,PLT,EOS%before and after processing by the three kinds of methods were statistically significant(P<0.05).Conclusion:Chylous disturbs the blood cell analysis of Hb,MCH, MCHC,PLT,the influence degree is associated with the severity of chylemia.The three methods of dilution,blank,replacement can eliminate the interference, but the method of dilution is easy to operate and can detect small interference,it has the value of popularization.