Experimental Research of PLLA/Pearl Layer Powder Composite Scaffolds to Repair Bone Defect

ZHU Lei; WANG Zhi-bin; HU Ning-min

doi:10.14033/j.cnki.cfmr.2015.22.001

Abstract：Objective:To prepare PLLA/pearl layer powder composite scaffolds in repairing bone defects in vivo degradation and biocompatibility, provideing the experimental basis for clinical application of bone repair.Method:Using the freeze drying method to prepare PLLA/pearl layer powder composite scaffolds,its shape characterization was measured by SEM,bracket of pore structure was measured by mercury injection apparatus,scanning method to collect data at low pressure mode.To choose 24 healthy adult New Zealand white rabbit as experimental objects,the selection of the rabbits were randomly divided into four groups:group A(PLLA scaffold materials),group B(PLLA/pearl layer stent group),group C(tiny particles bone group),group D(blank group).Through the establishment of middle 1 cm in bilateral radius as a bone repair bone defect model,to detect different composite materials stents defect.In different cages,for three consecutive days to give gentamicin to prevent infection and after 4,8,12 weeks to go imaging,histology,bone mineral density,graded by Lane-sandhu, and valuated of composite scaffolds into bone.Result:PLLA/pearl layer powder composite scaffold was prepared successfully,scanning were angled trapezium of the porous structure,we could see pearl layer powder evenly distributed on the hole wall and the edge, scaffold porosity basically were all between 88%-93%. Dual-energy X-ray absorptiometry combined postoperative we could see that after 4,8 weeks group B composite support score by X-ray and histological grading were slightly lower than those of group C(P<0.05),after 12 weeks there was no statistical significance between group B and group C(P>0.05).Conclusion:The self-made PLLA/pearl layer powder composite scaffolds has a good osteogenesis effect, and the results are provided the basis for clinical application.