The Effect of Knockdown ILK by shRNA on the Proliferation,Migration and Invasion of Human Glioma Cell U251
WANG Guang-yi
ZHENG Ke-bin
LI Chun-hui
Abstract：Objective:To investigate the change of tumorigenesis,proliferation,migration and invasion of human brain cells U251 after knockdown ILK by shRNA interference.Method:U251 were transfected with the specific ShRNA(ILK-PGFP-V-RS) vector(U251-si),and empty vector were constructed as a negative control(U251-N).Stable transfected cells were established.The expression of ILK Cyclin D1 E-cadherin Snail mRNA and its protein among the stable transfected cells were detected by RT-PCR,Western blot,respectively.Cell migration and invasion ability were detected by Transwell assay(Matrigel matrix per well were used in vasion assay).Result:The stable transfected cell lines U251,U251-si,U251-N were constructed. Conpared to the control cell,the anility of proliferation,migration,invasion were markedly inhibited in the cells(P<0.05).Conclusion:The inproliferation, migration and invasion are inhibited by knockdown ILK through shRNA in glioma cell U251,suggesting that loss of ILK may play a significant role in gliomagenesis.
Keywords：GliomashRNAILKE-cadherin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )