The Experimental Study of Juglandis Folia Extracts on the Isolated Perfused Frog Heart Activities
Abstract：Objective:To primarily discuss the influence of juglandis folia on cardiomotility and its mechanism of action through the experiments of effects of juglandis folia extracts on isolated frog heart irrigation.Method:110 frog hearts were selected and divided into 11 groups according to the random number table method:5 groups of effects square (normal control group,very low,low,medium and high juglandis folia extract concentration group) and 6 groups of mechanism square (normal control group,juglandis folia extract group,Atropine low concentration group,Atropine high concentration group,Atropine low concentration group+juglandis folia extract group,Atropine high concentration group+juglandis folia extract group).Corresponding liquid was added into frog heart perfusion tube of each group.Heart rate and heart contraction of isolated frog in each group before and after medication were recorded by BL-410 Biological Functional Experiment system.The results were compared and analyzed.Result:There were no significant differences in the heart rate between each juglandis folia group and normal control group(P>0.05).The value of heart contraction of each juglandis folia group after medication were significantly lower than that of normal control group(P<0.05 or P<0.01),and presented the heart contraction amplitude gradually decreased with the increase of the concentration of juglandis folia.The value of Atropine low concentration group+juglandis folia extract group was significantly lower than that of Atropine low concentration group (P<0.01).The value of Atropine high concentration group+juglandis folia extract group was significantly lower than that of Atropine high concentration group(P<0.01).The value of juglandis folia extract group,Atropine low concentration group+juglandis folia extract group and Atropine high concentration group+juglandis folia extract group were significantly lower than that of normal control group(P<0.01),but there were no significant differences in pairwise comparison among the three(P>0.05).Conclusion:Juglandis folia has no effects on heart rate,and it has certain inhibitory effect on cardiac contractility.Dose-effect relationship can be figured out in the inhibiting effect.The inhibiting effect on heart contraction may not be accomplished through M Receptor System.
Keywords：Juglandis folia extractsInhibitionIsolated frog heartCardiac contractility
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3-5 )