Study on the Radiosensitivity Heterogeneity of Different Metastatic Potential Cell Lines of Human Prostate Cancer and Its Mechanism
CHEN Dong-bo
YANG Li-ping
Abstract：Objective:To study the radiosensitivity heterogeneity of different metastatic potential cell lines of human prostate cancer PC-3M-2B4(low metastatic potential) and PC-3M-IE8(high metastatic potential) and Its mechanism.Method:To detect differences in PC-3M-2B4 and PC-3M-IE8 apoptotic ability,to detect the differences of apoptotic ability in PC-3M-2B4 and PC-3M-IE8 and the expression of apoptosis-related proteins Bcl-2,Bax and Caspase-3 by flow cytometry,fluorescence microscopy,electron microscopy and Western-blot.Result:The apoptosis rate of two cell PC-3M-2B4 and PC-3M-IE8l were irradiated with the extension of time increases.Compared to PC-3M-IE8,the apoptosis rate of PC-3M-2B4 was statistically significant(P<0.05).The expression of anti-apoptotic protein Bcl-2 decline with time-dependent,while the expression of pro-apoptotic proteins Bax and Caspase-3 was significantly increased. Conclusion:Human prostate cancer cell lines with different metastatic potential,its ability of transfer has related to the and radiosensitivity,those who have low transfer ability with high radiosensitivity.
Keywords：Prostate cancerHeterogeneityRadiosensitivityApoptosisCancer gene
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 15-17 )