Comparative Study of the Flow Cytometry Method and Magnetic Enzyme Linked Immunosorbent Assay for Detection of HLA-B27

ZHU Jian-ming

Abstract：Objective:To analyze the clinical value of flow cytometry method and magnetic enzyme linked immunosorbent assay for detection of HLA-B27 in paitients with ankylosing spondylitis(AS).Method:164 patients with suspected AS were chencked with the flow cytometry method and magnetic enzymelinked immunosorbent assay for detection of HLA-B27 from May 2010 to May 2013 in our hospital.The clinical diagnosis results being served as a reference,the sensitivity,specificity,accuracy,positive predictive value and negative predictive value of two methods were analyzed.Result:143 cases were diagnosed and 21 cases were excluded in 164 cases of suspected AS patients.The clinical diagnosis being severed as the reference standard,the sensitivity of flow cytometry method and magnetic enzyme linked immunosorbent assay were 96.5%,84.6%.The specificity of two methods were 71.4%,95.2%,and the accuracy was 93.3%, 86.0%.There were significant differences between two methods(χ2=11.820,P=0.001;χ2=4.286,P=0.038;χ2=4.725,P=0.030).About the positive predictive value and negative predictive value of two methods there were no significant differences between two methods (χ2=2.887,P=0.090;χ2=0.625,P=0.419). Conclusion:Magnetic enzyme linked immunosorbent assay and flow cytometry method have their respective advantages and disadvantages,and two methods together have an important reference value for AS.