Real-time Fluorescent Quantitative PCR for the Influence Factors of Hepatitis B DNA Testing
LAI Xin-hua
LV Na
PENG Yan-hui
Abstract：Objective:To explore effect of real-time fluorescent quantitative PCR detection of hepatitis B DNA,analyze the influence factors.Method:From January 2010 to January 2012 were between 300 patients with hepatitis b virus (HBV) infection,collect the blood specimens,by real-time fluorescent quantitative PCR detection method for hepatitis B DNA testing,the result of the inspection result and clinical diagnosis,this paper compares and analyzes the effect factors of test results is analyzed.Result:300 cases of hepatitis b infection in patients with blood specimens,using real-time fluorescent quantitative PCR of hepatitis B DNA test results and clinical diagnosis results of 276 cases of same (92.0%),misdiagnosis and misdiagnosed 24 cases,accounted for 8.0%,the main influence factors including laboratories,specimen and nucleic acid extraction method,etc.Conclusion:The real-time fluorescent quantitative PCR to detect HBV DNA,has higher diagnosis rate,but the influence on test results of many factors,the need to strengthen the attention,to decrease the rate of missed diagnosis and misdiagnosis,provide effective support for treatment.
Keywords：Real time fluorescence quantitative PCRHBV DNAFactors affecting
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 63-64 )