The Influence of Vitro Co-culture Environment on Canine Adipose Tissue Derived Stem Cell and Oral Keratinocyte
ZHANG Qin
TIAN Yu-jing
CHENG Li
Abstract：Objective:To investigate the influence of vitro co-culture environment on the migration and proliferation patterns of canine adipose-tissue derived stem cell and oral keratinocytes.Method:Obtain canine ADSC,OK and make the identification.Seed the two cell lines onto a scaled dish,record the migration rate of the two cell lines in co-culture environment,and compare it with the record in monoculture environment.Collect the supernatant of the two cell lines,and add it to the other’s culture medium to simulate a co-culture environment.Detect the changes in cell proliferation curves through MTT assay.Result:The migration rate of the ADSC and OK improved in co-culture environment.The proliferation curves of the two cell line were steeper in co-culture environment than in monoculture environment.Conclusion:In vitro co-culture environment,ADSC,OK exhibit mutual promotion,mutual attraction,collaborative proliferation situation,cell migration rate and proliferation rate are improved.They can be used together for the construction of the tissue engineering oral mucosa.
Keywords：Adipose tissue derired stemOral keratinocyteThe tissue engineeringCo-culture environment
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-2,3 )