The Mult-Omics Analysis of THP-1-Derived Macrophages Stimulated by 630 nm Light Emitting Diodes and Lipopolysaccharide
REN Dandan
BI Wanying
XIE Wenting
WU Jiaxin
SONG Wuqi
ZHANG Fengmin
LIU Hailiang
Abstract：LED,as a form of Low-Level Light Therapy(LLLT),have been proven effective in treating diseases such as lung injury,tendinopathy,nerve damage,and rheumatoid arthritis.This study uses 630 nm LED red light as the light source to analyze the mechanism of action of 630 nm LED red light on macrophages derived from human monocytic leukemia cells(THP-1)stimulated with lipopolysaccharides(LPS).THP-1 cells were treated with LPS and irradiated with 630 nm LED red light at an intensity of 28.8 J/cm2.The mRNA expression levels of pro-inflammatory factors CXCL9,CXCL10 and CXCL11 were then measured.Differentially expressed proteins were screened using non-quantitative proteomics and phosphoproteomics analysis methods.Gene Ontology(GO)and KEGG enrichment analyses were performed.Through STRING online database( Cytoscape software is used to analyze protein interactions.The results showed that the mRNA expression levels of CXCL9,CXCL10 and CXCL11 in the LED-irridated THP-1 cells were significantly reduced compared to the control group.Proteomic analysis revealed that after 630 nm LED red light irradiation of macrophages,23 differentially expressed proteins were significantly enriched in pathways such as transcriptional regulation complexes and viral defense response regulation.Phosphoproteomics results showed that the modification levels of 178 sites were significantly enriched in pathways such as nuclear spots,cadherin binding,protein signal transduction,positive regulation of protein localization,and actin cytoskeleton.Combined with multi-omics analysis,we speculate that the 630 nm LED red light may exert anti-inflammatory effects through multiple pathways.
Keywords：light-emitting diodeTHP-1630 nm red lightproteomicsphosphorylated proteomicsphotobiological regulation
Publication Date：2025-04-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 39-48 )