Changes in Serum Levels of VEGF and NOS in Patients with Retinal Light Injury and Their Diagnostic Efficacy

XU Xiaoyan; XU Xiaoshan

doi:10.13919/j.issn.2095-6274.ZHJY202505015

Abstract：Objective To investigate the changes in serum levels of vascular endothelial growth factor(VEGF)and nitric oxide synthase(NOS)in patients with retinal light injury and their diagnostic efficacy for the condition.Methods A total of 168 patients diagnosed with retinal light injury from January 2022 to December 2024 were included in this study(light injury group).Patients were subdivided based on the severity of retinal le-sions into mild(56 cases),moderate(56 cases),and severe(56 cases)subgroups.Additionally,40 healthy individ-uals served as the control group.Serum VEGF levels were measured using enzyme-linked immunosorbent assay(ELISA),while serum NOS levels were assessed using colorimetric methods.Pearson correlation analysis was employed to evaluate the correlation between VEGF and NOS levels.The diagnostic efficacy of VEGF and NOS for retinal light injury was analyzed using receiver operating characteristic(ROC)curves.Results Serum levels of VEGF and NOS in patients with retinal light injury were significantly higher than those in the normal control group,with levels increasing progressively with the severity of retinal light injury(P＜0.001).Pearson correlation analysis indicated a positive correlation between VEGF and NOS(r=0.765,P＜0.001).The area under the curve(AUC)for VEGF was 0.912,with a sensitivity of 86.70％and specificity of 92.50％.The AUC for NOS was 0.843,with a sen-sitivity of 78.30％and specificity of 80.00％.Both VEGF and NOS demonstrated high diagnostic value for retinal light injury,with VEGF exhibiting superior diagnostic accuracy compared to NOS(Youden index:0.792 vs.0.583).Conclusion Serum levels of VEGF and NOS are significantly elevated in patients with retinal light injury and are positively correlated with disease severity.Both markers possess high diagnostic value for reti-nal light injury,with VEGF showing superior diagnostic efficacy.VEGF may serve as a potential biological marker for clinical auxiliary diagnosis and disease assessment.