Study on the Differential Expression of Ki67 Clones in Different Tumor Tissues
WU Lianping
LIN Yuqiong
LIN Qinghua
Abstract：Objective To investigate the expression differences of Ki67 antibodies with different clone numbers in various tumor tissues.Methods A total of 45 paraffin-embedded tissue specimens from three types of tumors were collected,with 15 specimens from each tumor type.A multi-tissue array was prepared.Immunohis-tochemical staining was performed using three different clones of Ki67:MIB-1,SP6,and MXR002,with all other conditions held constant.Normal tissue controls included tonsil,appendix,and adipose tissue.Results The proliferation indices of Ki67(MIB-1)in breast cancer,lung cancer,and cervical cancer were 66.7%(10/15),80%(12/15),and 86.7%(13/15),respectively,with weak to moderate staining intensity.The proliferation indices of Ki67(SP6)in breast cancer,lung cancer,and cervical cancer were 60%(9/15),73.3%(11/15),and 73.3%(11/15),respectively,with light to moderate staining intensity.In breast cancer,lung cancer,and cervical cancer,Ki67(MXR002)showed a proliferation index of 100%(15/15),with strong staining intensity,which was significantly higher than that of Ki67(MIB-1)and Ki67(SP6)(P<0.05).In normal tonsil and appendix tissues,Ki67(MXR002)also exhibited higher staining intensity and proliferation index compared to Ki67(MIB-1)and Ki67(SP6)(P<0.05).In adipose tissue samples,Ki67(MIB-1)antibody exhibited nonspecific staining,while Ki67(MXR002)and Ki67(SP6)antibodies did not show this phenomenon.Conclusion Ki67(MIB-1)and Ki67(SP6)have lower sensi-tivity,with Ki67(MIB-1)showing nonspecific staining in adipocytes.Therefore,Ki67(MXR002)is recommended for immunohistochemical staining.
Keywords：Ki67CarcinomaImmunohisto-chemistry
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 26-30 )