Effects of tar, nicotine and rosuvastatin on expression of EPCR in human umbilical vein endothelial cells

WANG Zhen; WEI Yujie; LAI Bin; LIU Huiliang

doi:10.13919/j.issn.2095-6274.2016.04.005

Abstract：Objective To explore the effects of tar, nicotine and rosuvastatin on expression of endothelial cell protein C receptor (EPCR) in human umbilical vein endothelial cells (HUVECs).Methods HUVECs were isolated and cultured in vitro, and were randomly divided into control group, 50 mg/L tar group, 10-4 mol/L nicotine group, 10-5 mol/L rosuvastatin at passage 4 to 6. All groups were incubated for 6 h and 24 h, then collected the HUVECs. Flow cytometry and quantitative real time polymerase chain reaction (qRT-PCR) were respectively used to detect and compare expression of EPCR protein and the EPCR level of messenger ribonucleic acid (mRNA) of in each HUVECs group.Results (1) Compared with control group at the same time point, expression of EPCR protein in 50 mg/L tar group after incubated for 6 h (t=29.513,P<0.001) and 24 h (t=41.759,P<0.001) were both increased, and the differences were statistically significant;but in 10-4 mol/L nicotine group and 10-5 mol/L rosuvastatin group had no obvious change. (2) Compared with control group at the same time point, expression of EPCR mRNA in 50 mg/L tar group after incubated for 6 h (t=20.753,P<0.001) and 24 h (t=19.897,P<0.001) were significantly increased; but there were no significant change in 10-4 mol/L nicotine group and 10-5 mol/L rosuvastatin group.Conclusions Tar has the effect of up-regulating the expression of EPCR in HUVECs, whereas nicotine and rosuvastatin have no effect on the expression of EPCR in HUVECs.