Study on plasma concentration of rutin in extract of persimmon leaves in rats by HPLC and its pharmacokinetics
CHEN Yangfang
Abstract：Objective To establish a high performance liquid chromatography (HPLC) method for measuring the concentration of rutin in extract of persimmon leaves in rats, and to study its pharmacokinetics.Methods Blood sample were collected through venous plexus in rat eye at different time after oral administration of the extract of persimmon leaves. After liquid extraction with methanol, the concentration of rutin in plasma was measured. Then pharmacokinetics parameters were calculated by 3P97 program.Results The results showed that plasma concentration-time curve of rutin in rats was coincided with two-compartment model, rutin was absorbed fast in rats. Peak concentration (Cmax) of rutin in rat plasma was (1.658±0.184) mg/L one hour after oral administration. The other main pharmacokinetic parameters were as follows: absorption rate constant (Ka) was (2.436±0.883)/h, distribution rate constant (α) was (1.002±0.372)/h, elimination rate constant (β) was (0.083±0.067)/h, elimination half time (t1/2β) was (9.418±7.256) h, area under the concentration-versus-time curve (AUC0-12) was (9.628±2.281) mg·h/L.Conclusions HPLC method is accurate and sensitive, it is suitable for the pharmacokinetic study of rutin in extract of persimmon leaves in rats.
Keywords：rutinextract of persimmon leavespharmacokineticsHPLC
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 568-571 )