Experimental study on the protective effect of different ischemic postconditioning scheme in rabbits with crush syndrome

WANG Jinqiang; LIU Ziquan; DING Hui; FAN Haojun; DONG Wenlong; SONG Meijuan; HOU Shike

doi:10.13919/j.issn.2095-6274.2015.05.003

Abstract：Objective To screen the best time-window of the protective effect of different ischemia postconditioning ( IPost) scheme on rabbits with crush syndrome ( CS) injury.Methods 48 healthy Japanese white rabbits were selected and randomly divided into control group ( S group) and 5 groups of different IPost scheme ( IPA~IPE group) , 8 rabbits each.CS model was established, and femoral arteries and veins were separated in the groin of both lower limbs before releasing of pressure.After decompression, the ar-teries of IPA group were treated with bulldog climp clamping for 5 s, then releasing 5 s, and repeated for 5 cycles (5 ×5 s/5 s), IPB group was 5 ×30 s/30 s, IPC group was 5 ×1 min/1 min, IPD group was 5 ×5 min/5 min, IPE group was 3 ×10 min/10 min, obser-vation lasted 24 h after releasing of pressure.The concentration of serum creatine kinase ( CK) , myoglobin ( Mb) and neutrophil gela-tinase-associated lipocalin (NGAL) were detected before establishment of CS model, immediateness after decompression and 2, 6, 12, 18 and 24 h after decompression.Results Compared with S group, the concentration of serum CK, Mb and NGAL were reduced in all Ipost groups after decompression.All indexes of groups of IPC and IPD in 6, 12, 18, 24 h after decompression was statistical significant in comparison with S group (P<0.005);All indexes of IPC group were lower than IPD group in each time point after decompression, but there was no significant difference.Conclusions Giving 1-5 min remote ischemia postconditioning for 5 cycles before reperfusion to rabbits with crush syndrome may produce effective protection to organism injuries; among these groups, IPC group ( 5 ×1 min/1 min) showed the most obvious effect.