The Relationship Between POU5F1 and PBX1 Expression in Diffuse Large B-Cell Lymphoma Tissues and Patient Clinicopathological Characteristics and Prognosis
WANG Man
SU Ailing
HU Mingqiu
YANG Jie
YANG Dan
ZHANG Xiuqun
Abstract：Objective To explore the relationship between the expression of POU domain class 5 transcription factor 1(POU5F1)and pre-B-cell leukemia homeobox transcription factor 1(PBX1)in diffuse large B-cell lymphoma(DLBCL)tissues and the clinicopathological features and prognosis of patients.Methods A total of 102 patients with DLBCL admitted to Nanjing First Hospital from March 2019 to February 2022 were selected as the research subjects.DLBCL tissues and adjacent tissues obtained surgically were designated as the DLBCL group and the adjacent tissue group,respectively.The expression of POU5F1 and PBX1 was detected by immunohistochemistry.Clinicopathological data of the patients were collected,and the relationship between POU5F1/PBX1 expression and the clinicopathological characteristics of DLBCL patients was analyzed using the χ2 test.All patients were followed up for 3 years and were divided into a survival group(71 cases)and a death group(31 cases)based on their survival status at the end of follow-up.Univariate and multivariate Cox regression analyses were used to analyze the factors influencing the prognosis of DLBCL patients.Results The positive expression rates of POU5F1 and PBX1 in the DLBCL group were higher than those in the adjacent tissue group(P<0.05).DLBCL patients with clinical stage Ⅲ-Ⅳ,bone marrow infiltration,or extranodal involvement≥2 had higher positive expression rates of POU5F1 and PBX1 than patients with clinical stageⅠ-Ⅱ,no bone marrow infiltration,or extranodal involvement<2(P<0.05).The positive expression rates of POU5F1 and PBX1 in the death group were higher than those in the survival group(P<0.05).Multivariate Cox regression analysis showed that bone marrow infiltration(HR=2.250,95%CI:1.544-3.278),extranodal involvement≥2(HR=2.330,95%CI:1.590-3.415),positive expression of POU5F1(HR=2.804,95%CI:1.854-4.240),and positive expression of PBX1(HR=2.465,95%CI:1.662-3.655)were risk factors for death in DLBCL patients(P<0.05).Conclusion POU5F1 and PBX1 are aberrantly highly expressed in DLBCL tissues.Their expression is associated with clinical stage,bone marrow infiltration,and the number of extranodal involvement sites,and they may serve as potential prognostic biomarkers for DLBCL patients.
Keywords：diffuse large B-cell lymphomaPOU domain class 5 transcription factor 1pre-B-cell leukemia homeobox transcription factor 1clinicopathological characteristicsprognosis
Publication Date：2026-02-20
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：6( 256-261 )