Value of Nano-Carbon Tracer Sentinel Lymph Node Biopsy and miR-3613,miR-29a in Risk Stratification of Early Endometrial Cancer
ZHAO Chenyang
PENG Lixiu
TAN Chen
ZHANG Wei
YANG Yajuan
HE Changhui
GUO Litong
Abstract：Objective To investigate the value of nano-carbon tracer sentinel lymph node(NCT-SLN)biopsy and microRNA(miR)-3613,miR-29a in the risk stratification of early endometrial cancer(EC).Methods A 1:1 matched case-control study was conducted.Patients with early intermediate-high risk EC admitted to Chenzhou No.1 People's Hospital from October 2022 to July 2024 were selected as the observation group(146 cases),and patients with early low-risk EC during the same period were selected as the control group(100 cases).General data,conventional tumor markers[carbohydrate antigen 125(CA125),carcinoembryonic antigen(CEA),human epididymis protein 4(HE4)],NCT-SLN positive rate,and levels of miR-3613 and miR-29a were compared between the two groups.The NCT-SLN positive rate and levels of miR-3613 and miR-29a were compared among observation group patients with different pathological characteristics.Contingency correlation coefficient was used to analyze the correlation between NCT-SLN positivity and clinicopathological characteristics.Spearman rank correlation was used to analyze the correlation of miR-3613 and miR-29a with clinicopathological characteristics.ROC curve analysis was used to evaluate the predictive value of miR-3613,miR-29a,NCT-SLN,CA125,CEA,and HE4 for early EC risk stratification.Results The observation group had higher age,serum CA125,CEA,HE4 levels and NCT-SLN positive rate than the control group,and lower serum miR-3613 and miR-29a levels than the control group(P<0.05).Intermediate-high risk EC patients with tumor diameter≥5 cm,low differentiation,FIGO stage Ⅱ,and deep myometrial invasion had a higher NCT-SLN positive rate and lower miR-3613 and miR-29a levels than those with tumor diameter<5 cm,moderate-high differentiation,FIGO stage Ⅰ,and superficial myometrial invasion(P<0.05).Correlation analysis showed that tumor diameter,FIGO stage,and myometrial invasion were positively correlated with NCT-SLN positive rate(P<0.001),and negatively correlated with miR-3613 and miR-29a(P<0.001).Differentiation degree was negatively correlated with NCT-SLN positive rate(P<0.001),and positively correlated with miR-3613 and miR-29a(P<0.001).ROC curve analysis showed that the AUC of M1 for predicting early EC risk stratification was 0.948,which was better than M2 and individual detection(P<0.05).Conclusion NCT-SLN biopsy combined with serum levels of miR-3613,miR-29a,CA125,CEA,and HE4 has certain predictive value for early EC risk stratification,which can serve as a new protocol for early clinical risk stratification and guide clinical decision-making.
Keywords：nano-carbon tracingsentinel lymph nodebiopsymiR-3613miR-29aendometrial cancerrisk stratification
Publication Date：2026-02-20
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：6( 186-191 )