Study on the Expression and Diagnostic Value of the HMGB1/TLR4 Signaling Pathway in Peripheral Blood Mononuclear Cells of Patients with Acute Myocardial Infarction Complicated by Lung Infection
JIANG Li
TAO Ling
HU Tao
WANG Xiaohong
REN Jun
Abstract：Objective To explore the expression and detection value of the high mobility group box 1/Toll-like receptor 4(HMGB1/TLR4)signaling pathway in peripheral blood mononuclear cell(PBMC)of patients with acute myocardial infarction(AMI)and lung infection.Methods A total of 173 patients with AMI admitted to the First Affiliated Hospital of Air Force Medical University from January 2023 to January 2025 were selected as the study subjects.They were categorized into an infection group(104 cases)and a non-infection group(69 cases)based on the occurrence of pulmonary infections during hospitalization.Pathogenic culture was performed on the patients,and those in the infection group were further divided into a severe group(26 cases)and a mild group(78 cases).The expression levels of HMGB1/TLR4 signaling pathway-related proteins in PBMCs were compared among patients with different infection severities and the control group.The Spearman test was used to analyze the correlation between the expression of HMGB1/TLR4 signaling pathway-related proteins and the severity of lung infection.The receiver operating characteristic curve was applied to analyze the evaluation value of HMGB1/TLR4 signaling pathway-related protein expression for the severity of AMI patients with lung infection.Logistic regression analysis was used to examine the relationship between the expression of HMGB1/TLR4 signaling pathway-related proteins and the aggravation of lung infection.Results The protein expression levels of HMGB1,nuclear factor-κB(NF-κB),TLR4,and myeloid differentiation factor 88(MyD88)in the severe and mild groups were higher than those in the non-infection group(P<0.05).The protein expression levels of HMGB1,NF-κB,TLR4,and MyD88 in the severe group were significantly higher than those in the mild group(P<0.05).The protein expression levels of HMGB1,NF-κB,TLR4,and MyD88 in AMI patients with lung infection were positively correlated with the severity of lung infection(P<0.05).The area under the curve for the combined detection of HMGB1,NF-κB,TLR4,and MyD88 protein expression levels in PBMCs for evaluating the severity of lung infection was greater than that of each indicator alone(P<0.05).HMGB1≥9.11,NF-κB≥1.59,TLR4≥11.23,and MyD88≥1.61 were risk factors for the aggravation of lung infection in AMI patients(P<0.05).Conclusion The expression of the HMGB1/TLR4 signaling pathway has evaluation value for the severity of lung infection in AMI patients.
Keywords：acute myocardial infarctionlung infectionperipheral blood mononuclear cellshigh mobility group box1Toll-like receptor 4
Publication Date：2025-11-20
Online Publishing Date：2025-11-24(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )