The Mechanism of the Effect of Tetrabromobisphenol A/Tetrachlorobisphenol A on Human Hepatocellular Carcinoma Cell Line Huh7 Cells
XIA Zhenyi
CHEN Bin
LI Xiaoyan
YUAN Hongxia
LIU Jia
LI Yan
Abstract：Objective To investigate the mechanism of action of different concentrations of tetrabromobisphe-nol A(TBBPA)and tetrachlorobisphenol A(TCBPA)on human liver cancer cell line Huh7,and to assess their health risks.Methods Huh7 cells were exposed to TBBPA and TCBPA solutions at concentrations of 10 ng/L,100 ng/L,and 1 000 ng/L,and compared with blank Huh7 control cells.Cell proliferation activity,lipid droplet observation,gene expression analysis,and transcriptome sequencing were used to evaluate cytotoxicity and gene expression changes.Results At a concentration of 10 ng/L,TBBPA and TCBPA promoted Huh7 cell proliferation,and the proliferation activity was(117.3±1.4)%and(127.6±1.5)%,re-spectively;at a concentration of 1 000 ng/L,TBBPA and TCBPA lead to an increase in lipid droplets in Huh7 cells,and TBBPA increased the proportion of late stage apoptosis in Huh7 cells to(11.3±0.5)%,while TCBPA increased the proportion of early stage apoptosis to(8.0±0.4)%.Transcriptome sequencing showed that TBBPA and TCBPA respectively regulated 149 and 171 key genes,among which the genes LDLR,HSD17B7,NSDHL,and CREB3L3 were significant upregulated,significantly affect-ing lipid metabolism and steroid biosynthesis pathways.Conclusion TBBPA and TCBPA have significant toxicity to Huh7 cells at high concentrations,affecting lipid metabolism and inducing cell apoptosis.This study provides a scientific basis for evaluating the safety of bisphenol A substitutes and suggests the potential health risks of these substitutes.
Keywords：Tetrabromobisphenol ATetrachlorobisphenol ACytotoxicityLipid metabolism
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 121-128 )