Expression of CBX7 mRNA and miR-21 in Bladder Cancer and their Diagnostic Value

HU Weiqiang; WANG Long; DU Yuefeng; GAN Weimin; ZHANG Liuyong; LIU Kunlong; ZHANG Zhenqi; KE Longlong; WANG Yantao

doi:10.3639/j.issn.2095-3097.2025.06.005

Abstract：Objective To investigate the expression of CBX7 and miR-21 in bladder cancer tissues,and analyze the diagnostic value of their combination in bladder cancer.Methods The cancer tissue samples and adjacent tissues of 120 bladder cancer patients who underwent surgical treatment in Xi'an Gaoxin Hospital from January 2021 to December 2023 were collected.The expressions of CBX7 mRNA and miR-21 were detected by real-time quantitative PCR,the expressions of CBX7 mRNA and miR-21 in bladder cancer tissues and adjacent tissues were analyzed.Pearson method was used to analyze the cor-relation between the expression of CBX7 mRNA and miR-21 in bladder cancer tissues.The ROC curve was used to analyze the diagnostic value of CBX7 mRNA and miR-21 expression in patients with bladder cancer.Results The expression of CBX7 mRNA in bladder cancer tissue was significantly lower than that in adjacent tissue(P<0.05),and the expression of miR-21 was significantly higher(P<0.05);The expression of CBX7 mRNA in bladder cancer tissues of patients with TNM stage Ⅲ-Ⅳ,low differentiation and lymph node metastasis was lower than that of patients with TNM stageⅠ-Ⅱ,moderately and high differen-tiation and no lymph node metastasis.The expression of miR-21 in bladder cancer tissues of patients with TNM stage Ⅲ-Ⅳ,low differentiation and lymph node metastasis was higher than that of patients with TMN stageⅠ-Ⅱ,moderately and high dif-ferentiation and no lymph node metastasis(P<0.05);The expression of CBX7 mRNA and miR-21 in bladder cancer tissue was negatively correlated(r=-0.188,P<0.05);The area under the ROC curve of CBX7 mRNA combined with miR-21 to diagnose bladder cancer was 0.945,and the sensitivity and specificity were 96.67%and 82.50%,respectively.Conclusion The expres-sion of CBX7 mRNA in bladder cancer tissue is down-regulated,and the expression of miR-21 is up-regulated.The combined detection of CBX7 mRNA and miR-21 is of high significance for the auxiliary diagnosis of bladder cancer patients.