Expression Levels and Significance of RANKL,PTGER2 and COX2 Genes in Peripheral Blood Mononuclear Cells of Patients with Knee Osteoarthritis
YANG Lijuan
ZHOU Yanping
YU Nan
SU Xia
CHI Shuhong
Abstract：Objective To analyze the expression levels and significance of receptor activator of nuclear factor-kappa B ligand(RANKL),prostaglandin E receptor 2(PTGER2)and cyclooxygenase-2(COX2)genes in peripheral blood mononuclear cells(PBMCs)from patients with knee osteoarthritis(KOA).Methods A total of 100 patients with KOA admitted to.General Hospital of Ningxia Medical University from January 2018 to December 2023 were.selected as the KOA group.Meanwhile,30 healthy volunteers were selected as the control group.The expression levels of RANKL,PTGER2,and COX2 genes in PBMCs of the two groups were detected.The Logistic regression model was used to analyze the relationship between the expression levels of RANKL,PTGER2 and COX2 genes and the risk of KOA.The diagnostic efficacy of the expression levels of RANKL,PTGER2 and COX2 genes for KOA was evaluated using the receiver operating characteristic curve.Results The expression levels of RANKL,PTGER2,and COX2 genes in the KOA group were significantly higher than those in the control group(P<0.05).Logistic regression analysis results showed that the expression levels of RANKL,PTGER2,and COX2 genes were related to the occur-rence of KOA(P<0.05).The area under the curve(AUCs)of RANKL,PTGER2,and COX2 genes for diagnosing KOA were 0.828,0.876 and 0.800.The AUC of combined diagnosis with the three was 0.970(95%CI:0.924-0.992),which was significantly greater than that of single diagnosis(Z=8.986,11.959,7.285,P<0.01).The cutoff value,sensitivity and specificity of combined diagnosis of KOA were 0.30,91.00%and 96.67%.Conclusion The expression levels of RANKL,PTGER2,and COX2 genes in patients with KOA are high,and significantly correlated with the risk of KOA.Combined detection can enhance the diagnostic efficacy.
Keywords：Knee osteoarthritisPeripheral blood mononuclear cellsReceptor activator of nuclear factor-kappa B li-gandProstaglandin E2 receptorCyclooxygenase-2Diagnostic efficacy
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 66-70 )