Correlation between Plasma miRNA-223 and miRNA-489-3p and Severity of Coronary Artery Lesion in Patients with Coronary Heart Disease
DU Peishan
SHI Miao
SHI Jinzheng
GAO Bo
WU Caihong
ZHAO Haimei
TIAN Yanzhen
Abstract：Objective To analyze the correlation between plasma microRNA-223(miRNA-223)and miRNA-489-3p and the severity of coronary artery lesion in patients with coronary heart disease.Methods Clinical data from 134 patients with suspected coronary heart disease who underwent coronary angiography(CAG)at Zhangjiakou First Hospital from January 2020 to June 2022 were collected.Patients were divided into acute myocardial infarction(AMI)group(n=36),unstable angina pectoris(UAP)group(n=49),stable angina pectoris(SAP)group(n=28),and non-coronary heart disease control group(n=21).Plasma levels of miRNA-223 and miRNA-489-3p were compared among the four groups.Gensini score was used to evaluate the severity of coronary artery lesion in patients with coronary heart disease,and the patients were divided into low score group(Gensini score＜20 points),middle score group(Gensini score of 20-40 points)and high score group(Gensini score＞40 points).The differences in various indicators were compared among patients with different Gensini scores.The correlation between levels of plasma miRNA-223 and miRNA-489-3p and Gensini score,platelet distribution width(PDW),mean platelet volume(MPV),nuclear factor-κB(NF-κB),and tumor necrosis factor-α(TNF-α)in patients with coronary heart disease was analyzed.Results The levels of peripheral blood PDW,MPV,NF-κB,TNF-α,and expression level of miRNA-223 in the four groups revealed AMI group＞UAP group＞SAP group＞control group(all P＜0.05),while the expression level of plasma miRNA-489-3p was manifested as AMI group＜UAP group＜SAP group＜control group(P＜0.05).The Gensini score in the three groups demonstrated AMI group＞UAP group＞SAP group(P＜0.05).With Gensini score as grouping basis,there were 20 cases in low score group,52 cases in middle score group,and 40 cases in high score group,and the expression level of plasma miRNA-223 and levels of peripheral blood PDW,MPV,NF-κB,and TNF-α showed low score group＜middle score group＜high score group(P＜0.05),while the expression level of miRNA-489-3p revealed low score group＞middle score group＞high score group(P＜0.05).Pearson correlation coefficient analysis showed that the expression level of plasma miRNA-223 in patients with coronary heart disease was significantly positively correlated with Gensini score,peripheral blood PDW,MPV,NF-κB,and TNF-α(P＜0.05),and the expression level of plasma miRNA-489-3p was negatively correlated with the above indicators(P＜0.05).Conclusion Plasma miRNA-223 up-regulation and miRNA-489-3p down-regulation may promote the progression of coronary artery lesions,and its role mechanism may be related to the regulations of platelet activity and inflammatory response,which may open up a new direction for the clinical treatment of coronary heart disease.
Keywords：Coronary heart diseaseCoronary artery lesionmiRNA-223miRNA-489-3p
Publication Date：2025-02-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 46-51 )