Evaluation Value of miR-103 and miR-545 Expression in Triple-Negative Breast Cancer Tumor Tissue in Disease Progression and Prognosis
MA Jiequn
LI Suoni
LI Qian
BAI Jie
LI Xintao
Abstract：Objective To study the evaluation value of miR-103 and miR-545 expressions in triple-negative breast cancer(TNBC)tumor tissues in disease progression and prognosis.Methods A total of 103 patients with TNBC(TNBC group)and 52 patients with benign breast diseases(CON group)who were treated in Shaanxi Cancer Hospital from January 2019 to December 2021 were selected as the research subjects.Real-time fluorescence quantitative PCR was used to detect the expression of miR-103 and miR-545 in tumor tissues and adjacent tissues of the TNBC group and breast biopsy tissues of the CON group.Compare differences in the expression of miR-103 and miR-545 in tumor tissues in different clinicopathological characteristics of TNBC patients.The ROC curve was used to analyze the effectiveness of combined detection of miR-103 and miR-545 in tumor tissue in the TNBC group in predicting death of TNBC.Multivariate Cox regression was used to analyze the risk factors for death of TNBC.Kaplan-Meier survival models analyzed the relationship between the expression of miR-103 and miR-545 in tumor tissue and survival time in the TNBC group.Results The expression of miR-103 and miR-545 in the tumor tissue of the TNBC group was lower than that of the breast biopsy tissue of the control group and the adjacent tissue of the TNBC group(P＜0.05).The expression of miR-103 and miR-545 in TNBC patients with histological grade 3,Ki-67 index ≥60％,neurovascular invasion,primary tumor T stage T3-T4,regional lymph nodes N2-N3,distant metastasis,and TNM stage Ⅲ-Ⅳ was significantly lower than histological grade 1-2,Ki-67 index＜60％,no neurovascular invasion,primary tumor T stage T1-T2,regional lymph nodes N0-N1,no distant metastasis,and TNM stage Ⅰ-Ⅱ(P＜0.05).The sensitivity,specificity,and AUC of miR-103 combined with miR-545 in predicting TNBC death were higher than those of miR-103 and miR-545 alone.miR-103＜0.87,miR-545＜0.64,histological grade 3,Ki-67 index≥60％,neurovascular invasion,primary tumor T stage T3-T4,regional lymph nodes N2-N3,distant metastasis,and TNM stage Ⅲ-Ⅳ are independent risk factors for death in TNBC patients(P＜0.05).The median survival time of TNBC patients with tumor tissue miR-103＜0.87 and miR-545＜0.64 was significantly lower than that of patients with miR-103 ≥0.87 or miR-545 ≥0.64(median survival 28.94±5.62 months vs.37.26±6.34 months,Log rank=19.103,P＜0.05).Conclusion miR-103 and miR-545 expression in TNBC patients is significantly reduced,which may have certain clinical value in the evaluation of TNBC condition and prognosis.The combined detection of the two can improve the clinical value in the evaluation of TNBC mortality.
Keywords：Triple-negative breast cancermiR-103miR-545
Publication Date：2025-02-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 20-24,39 )