Effects of SDF-1 Expression on Proliferation,Invasion of Hepatocellular Carcinoma Cells and Tumorigenesis in Nude Mice
LI Ying
YANG Ni
ZHANG Yaojun
ZHANG Xiaoxia
GUO Rui
MA Shuhong
WANG Xiaoli
Abstract：Objective To investigate the effects of stromal cell-derived factor 1(SDF-1)expression on hepatocellular carcinoma(HCC)cell proliferation,malignant invasion and tumorigenesis in vivo.Methods shRNA targeting SDF-1 was transfected into HepG2 cells,and the cells were randomly divided into the Control group,shRNA-NC group,and SDF-1-shRNA1 group.Clone formation assays were performed to evaluate cell proliferation.Cell invasion was assessed using Transwell assays.Western blot was used to detect the protein expression levels of Ki67,Survivin,Vimentin,E-cadherin,and N-cadherin.A nude mouse transplantation model was established to compare tumor proliferation abilities between the Control and SDF-1-shRNA1 groups,and the number of Ki67-and VEGF-positive cells was determined by immunohistochemistry.Results Compared with the Control group,the SDF-1-shRNA1 group exhibited significantly decreased clone formation rates(P<0.05),Ki67 and Survivin protein levels(P<0.05),invasive cell counts(P<0.05),and Vimentin and N-cadherin protein levels(P<0.05).The E-cadherin protein levels were significantly increased(P<0.05).In vivo,the SDF-1-shRNA1 group showed reduced tumor volume and weight(P<0.05)and decreased numbers of Ki67-and VEGF-positive cells(P<0.05).Conclusion Interference with the SDF-1 gene inhibits HepG2 cell proliferation and invasion.
Keywords：Liver cancerSDF-1ProliferationInvasion
Publication Date：2025-01-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 204-209 )