Dihydroartemisinin Antagonizes Ang Ⅱ-Induced Transdifferentiation of Endometrial Epithelial Cells by Regulating Autophagy
LI Ling
SONG Yujie
PAN Yun
QIN Yan
GENG Xiao
SHAN Tieying
Abstract：Objective To investigate the effect of dihydroartemisinin on the transdifferentiation and autophagy protein expression of endometrial epithelial cells(EECs)induced by angiotensin Ⅱ(Ang Ⅱ),and to explore the mechanism of dihydroartemisinin antagonizing endometrial fibrosis.Methods Human primary EECs were obtained and divided into the following groups:normal control group,Ang Ⅱ group,dihydroartemisinin group and rapamycin group.Normal control group:cell incubation solution,Ang Ⅱ group:cell incubation solution+10-6mol/L Ang Ⅱ,dihydroartemisinin group:cell incubation solution+10-6mol/L Ang Ⅱ+100 ng/mL dihydroartemisinin,rapamycin group:cell incubation solution+10-6mol/L Ang Ⅱ+10-7mol/L rapamycin.The intervention time of each group was 24 hours.Measure the proliferation activity of cells in each group;ELISA measures the secretion of typeⅠ and typeⅢ collagen by cells;Immunoblotting was used to evaluate the levels of transdifferentiation proteins E-cad and α-SMA,autophagy proteins LC3-Ⅱ/Ⅰ,Beclinl and p62.Results The cell proliferation activity,secretion levels of typeⅠ,typeⅢ collagen,protein expression level of α-SMA,p62 in the Ang Ⅱ group were obviously higher than those in the normal control group(all P＜0.05),while E-cad,LC3-Ⅱ/Ⅰ and Beclinl were obviously lower than those of the normal control group(both P＜0.05);The cell proliferation activity,secretion content of type Ⅰ,type Ⅲ collagen,protein expression levels of α-SMA,p62 in dihydroartemisinin group were obviously lower than those in the AngⅡ group(all P＜0.05),while E-cad,LC3-Ⅱ/Ⅰ and Beclin1 were obviously higher than those of the Ang Ⅱ group(both P＜0.05);The cell proliferation activity,secretion content of typeⅠ,typeⅢ collagen,protein expression level of α-SMA,p62 in dihydroartemisinin group were obviously higher than those in rapamycin group(all P＜0.05),while E-cad,LC3-Ⅱ/Ⅰ and Beclin1 were obviously lower than those of the rapamycin group(both P＜0.05).Conclusion Dihydroartemisinin can reduce the proliferation activity of EECs induced by Ang Ⅱ and the secretion of typeⅠ and typeⅢ collagen,inhibit cell transdifferentiation,which is closely related to its ability to increase autophagy levels.
Keywords：DihydroartemisininAngiotensin ⅡEndometrial epithelial CellsCell transdifferentiationAutophagy
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1733-1737,1752 )