Effect of miR-10 Regulation of Notch Pathway on Hypoxia-Induced Retinal Ganglion Cell Injury

HUANG Yifan; LI Shanshan; YE Jianrong

doi:10.3639/i.issn.2095-3097.2024.10.037

Abstract：Objective To explore the effect of miR-10 on hypoxia-induced retinal ganglion cell(RGC)damage by regulating the Notch pathway.Methods RGC-5 cells were randomly divided into three groups:control group,miR NC group,and miR-10 group.RGC-5 cells in the miR NC group and miR-10 group were treated with hypoxia for 24 hours.RGC-5 cells were transfected with miR NC and miR-10 mimic,respectively,for 24 hours.The viability of RGC-5 cells was assessed using the MTT assay.The levels of inflammatory factors in the culture medium of RGC-5 cells were analyzed by enzyme-linked immunosorbent assay.The apoptosis of RGC-5 cells was analyzed by in situ end labeling.The targeting relationship between miR-10 and Notchl was analyzed by dual-luciferase reporter gene assay.The protein expression levels of Notch pathway proteins in RGC-5 cells were analyzed by Western blot.Results Compared with the control group,the proliferation activity of RGC-5 cells in the miR NC group was decreased,the apoptosis rate was increased,the levels of TNF-α,IL-1 β and IL-6 were increased,and the expression of Notch1 and hairy and enhancer of split 1(Hes1)protein was decreased(all P＜0.05).Compared with the miR NC group,the proliferation activity of RGC-5 cells in the miR-10 group was increased,the apoptosis rate was decreased,the levels of TNF-α,IL-1 β and IL-6 were decreased,and the expression of Notch1 and Hes1 protein was increased(all P＜0.05).miR-10 has a targeted binding domain with Notch1.Conclusion Up-regulation of miR-10 can reduce hypoxia-induced retinal ganglion cell damage,and this process is related to the regulation of the Notch pathway by miR-10.