Correlation between the Expression of Toll-like Receptor 2 mRNA in Peripheral Blood and the Changes of Th1/Th2/Th17 Cytokines and Prognosis in Patients with Multidrug-Resistant Tuberculosis
CHENG Xiaohui
TANG Zheng
LI Xinqin
LI Fei
LI Jie
YU Lei
LIN Qing
Abstract：Objective To investigate the relationship between the peripheral blood Toll-like receptor 2(TLR2)mRNA expression,T helper cell(Th)1/Th2/Th17 cytokine changes,and prognosis in multidrug-resistant tuberculosis(MDR-TB)patients.Methods A total of 30 MDR-TB patients,30 drug-susceptible tuberculosis(DS-TB)patients,and 30 healthy individuals with positive tuberculin pure protein derivative(PPD+)test results were included from November 2021 to May 2023.TLR2 mRNA levels in peripheral blood were detected by fluorescence quantitative PCR.Flow cytometry was used to measure CD4 cell ratio,T-bet+CD4+cell ratio,interleukin-4(IL-4),IL-17,and interferon-γ(IFN-γ)levels.Multivariate Logistic regression analysis was conducted to identify factors influencing MDR-TB prognosis.Results The CD4 cell ratio and IFN-γ levels in the PPD+group were significantly higher than those in the DS-TB and MDR-TB groups,while the IL-4,IL-17,and TLR2 mRNA levels were significantly lower(P＜0.05).In MDR-TB patients,the CD4 cell ratio and IFN-γ levels in the good prognosis group were significantly higher than those in the poor prognosis group,while IL-4,IL-17,and TLR2 mRNA levels were significantly lower(P＜0.05).Increased CD4 cell ratio and IFN-γ levels were protective factors,while elevated IL-4,IL-17,and TLR2 mRNA levels were risk factors for poor prognosis(P＜0.05).Conclusion High expression levels of TLR2 mRNA,IL-4,and IL-17 in peripheral blood are risk factors for poor prognosis in MDR-TB patients,while high CD4 cell ratio and IFN-γ levels serve as protective factors.Monitoring these biomarkers may have clinical significance in evaluating the prognosis of MDR-TB.
Keywords：TuberculosisDrug-resistant tuberculosisToll-like receptor 2T helper cell 1T helper cell 17Cytokine
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1651-1655 )