The Differential Value of IL-6,TLR-2 and CD14 Expression in Peripheral Blood of Patients with Severe Pulmonary Infection for Different Pathogenic Bacteria

SHI Youxiang; ZHANG Qiang; WANG Xiaoran

doi:10.3639/i.issn.2095-3097.2024.10.006

Abstract：Objective To explore the expression levels of interleukin-6(IL-6),Toll-like receptor 2(TLR-2),and leukocyte differentiation antigen-14(CD 14)in the peripheral blood of patients with severe pulmonary infection,and analyze their identification value for different pathogenic bacteria.Methods 91 patients(severe pulmonary infection)admitted to o Haian people's hospital(September 2021 to August 2023)were selected as observation group,during the same period,55 healthy persons(underwent physical examination)were selected as control group.The expression levels of IL-6,TLR-2,and CD 14 in the peripheral blood of the two groups were detected by enzyme-linked immunoassay,chemiluminescence method.The levels were compared among different pathogens,and receiver operating characteristic(ROC)curves were used to analyze the identify identification value of peripheral blood IL-6,TLR-2,and CD 14 expression in patients with severe pulmonary infection for different pathogenic bacteria.Results The levels of peripheral blood IL-6,TLR-2,and CD14 in the observation group were higher than control group(P＞0.05).In terms of IL-6 level in peripheral blood,G bacteria,fungi and G+bacteria infected patients were decreased successively,and ptwo comparison differences were significant(all P＜0.05).In terms of TLR-2 level in peripheral blood,G bacteria infected patients were lower than that of G+bacteria and fungi infected patients(P＜0.05),and there was no statistical significance between G-bacteria infected patients and fungi infected patients(P＞0.05).There was no significant difference in the level of CD14 in peripheral blood among G bacteria,G+bacteria and fungi(P＞0.05).ROC curve analysis results showed that the combined area under the curve(AUC)value of IL-6 and TLR-2(0.880)was higher than that of IL-6(0.819)and TLR-2(0.750)in the identification of G-bacteria and fungi.In the identification of G bacteria and G+bacteria,the AUC value of IL-6 and TLR-2 combined identification(0.885)was higher than that of IL-6(0.820)and TLR-2 alone identification(0.783).The AUC value of IL-6 for G+bacteria and fungi was 0.749.Conclusion The expression of IL-6,TLR-2 and CD14 in peripheral blood of patients with severe pulmonary infection is abnormally elevated,and IL-6 and TLR-2 can be used to distinguish different pathogenic bacteria types.