Expression of miR-190-5p and PHLPP1 in Lung Adenocarcinoma Tissue and Their Correlation with Pathological Parameters and Prognosis

SONG Jingzhou; YU Yanling; ZHANG Yan

doi:10.3639/j.issn.2095-3097.2024.09.016

Abstract：Objective To investigate the expression of microRNA-190-5p(miR-190-5p)and PH domain-containing leucine-rich repeat protein phosphatase 1(PHLPP1)in lung adenocarcinoma(LUAD)tissue and their correlation with patho-logical parameters and prognosis.Methods A total of 93 patients with LUAD who were treated at our hospital were selected and divided into high and low expression groups based on the mean levels of miR-190-5p and PHLPP1 mRNA in LUAD tis-sues.Real-time quantitative polymerase chain reaction was used to detect the expression of miR-190-5p and PHLPP1 mRNA in LUAD tissues and adjacent non-cancerous tissues.Differences in miR-190-5p and PHLPP1 mRNA expression between different pathological parameters in LUAD tissues were analyzed.The Kaplan-Meier(K-M)method was applied to plot survival curves for patients with different levels of miR-190-5p and PHLPP1 mRNA expression.Results Compared with adjacent tissues,miR-190-5p expression increased in LUAD tissues,while PHLPP1 mRNA expression decreased(P＜0.05).A neg-atively correlated was observed between PHLPP1 and miR-190-5 in LUAD tissues(P＜0.05).The expression of miR-190-5p and PHLPP1 mRNA in LUAD tissues differed significantly across levels of differentiation,TNM stage,and lymph node metastasis(P＜0.05).Patients high miR-190-5p expression had a lower 3-year overall survival rate compared to those with low expression,while patients with high PHLPP1 mRNA expression showed a higher 3-year overall survival rate compared to those with low expression(P＜0.05).Conclusion High expression of miR-190-5p and low expression of PHLPP1 mRNA in LUAD tissue are associated with differentiation,TNM stage,lymph node metastasis,and poor prognosis.