Expression and Diagnostic Value of Serum miR-130b and miR-937-5p in Patients with Non-small Cell Lung Cancer
HE Yuanqiang
WAN Yufeng
XU Chuanqin
FU Ran
LI Yue
WANG Yi
LI Gang
ZHENG Yulong
Abstract：Objective To investigate the expression levels of serum miR-130b and miR-937-5p in patients with non-small cell lung cancer(NSCLC)and their clinical diagnostic value.Methods In total,100 patients with NSCLC admitted from November 2020 to November 2022 were selected as the NSCLC group,and 100 healthy people who underwent physical examination in our hospital during the same period were selected as the control group.The expression levels of miR-130b and miR-937-5p were detected by RT-qPCR.The correlation between the expression levels of serum miR-130b and miR-937-5p in NSCLC patients was analyzed by Pearson method.The diagnostic value of serum miR-130b and miR-937-5p in NSCLC pa-tients was analyzed by receiver operating characteristic(ROC)curve.Cox regression analysis was performed to explore the risk factors of NSCLC.Results Compared with those in the control group,the expression levels of miR-130b and miR-937-5p in NSCLC group were significantly increased(P<0.01).Correlation analysis showed that there was a positive correlation between expression levels of serum miR-130b and that of miR-937-5p in NSCLC patients(r =0.672,P<0.01).The serum levels of miR-130b and miR-937-5p in NSCLC patients with lymph node metastasis,TNM stage Ⅲ to Ⅳ,and low differentia-tion were significantly higher than those in NSCLC patients with no lymph node metastasis,TNM stageⅠ to Ⅱ,and moder-ate-to-high differentiation(P<0.05,P<0.01).The area under the ROC curve(AUC)of miR-130b and miR-937-5p in combination for the diagnosis was higher than that of the detection of Mir-130B and Mir-937-5p alone(Z = 20.819,P<0.001;Z =4.328,P =0.037).Multivariate Cox regression analysis showed that differentiation degree,and expression level of serum miR-130b and miR-937-5p were risk factors for NSCLC(P<0.01).Conclusion The expression levels of both ser-um miR-130b and miR-937-5p in patients with NSCLC were significantly increased,and the combination of miR-130b and miR-937-5p was of high value in the diagnosis of NSCLC.
Keywords：Carcinomanon-small cell lungmiR-130bmiR-937-5pClinicopathological featureCorrelationIn-fluencing factor analysisDiagnostic valueReceiver operating characteristic curve
Publication Date：2023-10-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 262-266 )