Gentiopicroside Promotes Osteogenic Differentiation of Osteoblasts by In-hibiting the Expression of miR-103-3p

ZHANG Liru; LIU Yuanhang

doi:10.3969/j.issn.2095-3097.2023.05.002

Abstract：Objective To investigate the effects of gentiopicrin(GPS)on proliferation and differentiation of osteo-blast MC3T3-E1,and to analyze its regulatory effect on miR-103-3p.Methods Mouse osteoblast cell line MC3T3-E1 was treated with different doses of GPS,and the cell proliferation activity was detected by MTT assay.The expression of miR-103-3p was detected by qRT-PCR.The expression levels of Runt-related transcription factor 2(RUNX2),osteoprotegerin(OPG)and osteocalcin(OCN)were detected by qRT-PCR and Western blot.Alkaline phosphatase(ALP)activity was detected with reagent kit.In the subsequent experiment,anti-miR-NC group,anti-miR-103-3p group,GPS-H+miR-NC group,and GPS-H+miR-103-3p group were set up to compare the cell proliferation activity,ALP activity,and the protein and mRNA ex-pression levels of OPG,OCN,RUNX2 among all groups.Results After GPS treatment,cell proliferation activity,ALP ac-tivity,and mRNA and protein expression levels of OPG,OCN,and RUNX2 increased,while miR-103-3p expression de-creased in a dose-dependent manner(P＜0.05).Compared with anti-miR-NC group,cell proliferation activity,ALP activity and mRNA and protein expression levels of OPG,OCN and RUNX2 in anti-miR-103-3p group increased(P＜0.05).Com-pared with GPS-H+miR-NC group,cell proliferation activity,ALP activity,and mRNA and protein expression levels of OPG,OCN and RUNX2 in GPS-H+miR-103-3p group decreased(P＜0.05).Conclusion GPS can promote the prolifera-tion and differentiation of MC3T3-E1 cells by down-regulating the expression of miR-103-3p,and miR-103-3p may be a poten-tial target for GPS treatment of fracture.