The expression of heat shock protein 70 in ex-vivo porcine liver biliary after catheter radiofrequency ablation

YANG Chao; LU Wei; MA Junpeng; YANG Jian; JIANG Fuqiang; DU Peng; ZHANG Jiacheng; XIE Peng; ZHANG Zhe

doi:10.3969/j.issn.2095-3097.2018.03.007

Abstract：Objective To study the expression of heat shock protein 70 ( HSP70) in biliary of adjacent ablation area while catheter radiofrequency ablation ( RFA) in ex-vivo porcine liver biliary under different power parameters. Methods Fifteen fresh ex-vivo porcine liver bile duct systems ( gallbladder and common bile duct preserved) filled with human bile were divided into 3 group ran-domly. Then, the RFA catheter was inserted into the hilar biliary through the common bile duct. All the ablation time were 120 s, the power of 10 W, 12 W, and 14 W were used for each group respec-tively. The expression of HSP70 in biliary of 1 cm,2 cm away from the distal pole were examined by immunohistochemical S-P method. Results ①The expression level of HSP70 in biliary of 1 cm a-way from the distal pole showed 4 cases in the first stage ( 26. 7％) , 6 in second stage ( 40％) , and 5 in third stage (33. 3％); The expression level of HSP70 in biliary of 2 cm away from the distal pole showed 10 cases in the first stage ( 66. 7％) , 4 in second stage ( 26. 7％) , and 1 in third stage (6. 6％);②The HSP70 expression level in biliary of 1cm away from the pole showed a positive cor-relation with the RFA power ( r=0. 755, P<0. 001) . Rank sum test showed statistical difference be-tween the HSP70 expression level in biliary of 1 cm and 2 cm away from the distal pole ( Z=-2. 334, P<0. 05) . Conclusion The HSP70 expression in biliary of adjacent RFA area could be induced by the catheater RFA. More HSP70 expressed with increased power while no more HSP70 expression were found in biliary of 2 cm away from the distal pole.