Ozone intraperitoneal injection prevents the depression by ameliorating microglia re-activation in depressed mice

LU Guofang; AN Jianxiong; MA Jun; QIAN Xiaoyan; WANG Yong; YANG Xiaorong

doi:10.3969/j.issn.2095-3097.2018.01.006

Abstract：Objective Explore the impact of ozone on depression model, including the behaviors, the number of microglia cell and inflammation cytokines (IL-1β,IL-6 and TNF-α) in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Methods Forty mice were randomly divided into 4 groups; stress+saline (10), stress+ozone (10), control+saline (10), control + ozone (10). The model mice were set in a special centrifuge tube for 1 hour per day, lasting for a week, ozone by intraperitoneal injection of control + ozone and stress+ozone. Giving ozone 3 days before depression model as a ozone previously intervention. Giving ozone after depression model as a ozone therapeutic intervention. Tail suspension test (TST), forced swimming test (FST), novelty suppressed feeding test (NSFT), open field test (OFT) were subjected to detect the behavior of each group. Using the immunohistochemical technology to detect the number of microglia cell in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Using reverse transcription polymerase chain reaction technology to detect the expression level of inflammatory cytokines IL-1β,IL-6 and TNF-a from hippocampus, prefrontal cortex and amygdala region. Results After ozone previously intervention, ozone could improve depression behaviors in mice, reduce the number of activated microglial cells in the prefrontal cortex and hippocampus, decrease the levels of inflammatory cyto-kines IL-1β, TNF-α. After ozone intervention, ozone could not improve the depression behaviors in mice. There was no significant difference in the number of activated microglial cells in the prefrontal cortex, hippocampus and amygdala between stress+saline group and stress+ozone group (P＞0.05), ozone treatment failed to reduce the levels of IL-1β and TNF-a in the prefrontal cortex and hippocampus. Conclusion Ozone intraperitoneal injection has some effects on preventing the formation of depression, but there is no therapeutic effects on depression.