Recent research on optimized mass spectrum analysis of human body fluids peptidome
GAO Lu
GU Yan
CHEN Feng
Abstract：Peptidome composition of human body fluid has gained more and more attention. Different human body fluids are excreta or secreta of different organs or cells in physiological or patho-logical status. By monitoring specific changes of peptidome composition in human body fluids, we can potentially be able to predict human health status, development of diseasess, curative effects of treatments and pharmaceutical toxicity and so on noninvasively early, sensitively and specifically. However, compositions of human body fluids are complicated, the body fluid systems are vulnerable to many factors, so it’ s a big challenge to concentrate, extract and separate peptidome composition which are of low molecular weight and measure specific peptidome composition accurately. Matrix-assi-sted laser desorption/ionization time-of-flight mass spectrometry ( MALDI-TOF MS) method is wide-ly used in researches of peptidome composition of human body fluids at present. Analysis of deviation in experiments and proposing of improved operation can increase accuracy when analyzing peptidome composition in human fluids. Analysing peptidome of human fluids by MALDI-TOF MS method is a new thought of examining human physical conditions clinically. Specific peptidome compositions in human fluids have the potential to be new biomarkers to monitor human physical condition of related body parts.
Keywords：Body fluidPeptidomeMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( MALDI-TOF MS) methodDeviationOptimize
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 60-64 )