The effect of lipoxin A4 on the proliferation of human hepatocellular carcinoma under hypoxia
MIAO Shuo
WANG Sumei
CHENG Xue
LI Yaofeng
MA Mingyang
LI Gang
WU Ping
Abstract：Objective To explore the effect of lipoxin A4 ( LXA4 ) on the proliferation of hu-man hepatocellular carcinoma ( HCC) under hypoxic condition. Methods Ectopic HCC mice mo-del was established by injection H22 cells subcutaneously. Immunohistochemistry assay was applied to detect the protein expression of Ki-67 and hypoxia-inducible factor-1 α ( HIF-1α) . HepG2 cells were cultured under 1% O2 to establish a hypoxic condition. 3-( 4,5-dimethyl-2-thiazolyl)-2,5-di-phenyl-2-H-tetrazolium bromide ( MTT) assay was used to measure cell proliferation rate. Results In 7 to 15 days after H22 cell transplantation,the subcutaneous tumor in mice could be observed. At all the time points, the tumor volume of LXA4 mice was significantly reduced (P<0. 05). At the same time, LXA4 significantly decreased the expression of Ki-67 and HIF-1αinside tumor tissue. In vitro study showed that 200 nmol/L LXA4 obviously inhibited HepG2 proliferation under 1% O2 . Conclusion LXA4 could inhibit the HCC growth under hypoxia.
Keywords：Lipoxin A4 (LXA4)Hepatocellular carcinoma (HCC)HypoxiaProliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 16-19 )