Simultaneous determination of six components such as baicalin in Huangdan Yinchen Keli by high performance liquid chromatography
YANG Dan
ZHU Xujiang
XU Xukun
YAO Shixia
ZHAO Jianbang
Abstract：Objective To establish high performance liquid chromatography (HPLC) me-thod for the simultaneous determination of baicalin, baicalei, wogonoside, wogonin, oroxylin A and oroxylin A-glucoronide in Huangdan Yinchen Keli by HPLC. Methods HPLC was adopted by using an XB-C18 column (4. 6 mm×250 mm, 5 μm) with methanol-0. 1% phosphoric acid as the mobile phase. The flow rate was 1. 0 mL/min and the sample size was 10 μL. The determine wave length was 278 nm and the column temperature was kept at 30℃. Results The six components in Huangdan Yinchen Keli reached baseline separation within 40 minutes. Their relationship between the concentration and the peak areas was linear. The relative standard deviation ( RSD) of repeata-bility, precision and recovery were all less than 3. 0%. Conclusion This method is rapid, simple, reliable and it can simultaneously measure the content of baicalin, baicalei, wogonoside, wogonin, and oroxylin A, oroxylin A-glucoronide in Huangdan Yinchen Keli.
Keywords：Huangdan Yinchen KeliBaicalinBaicaleiHigh performance liquid chroma-tography ( HPLC)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 206-209 )