Extraction of total flavonoids from Sedum spectabile Boreau and its α-glucosidase inhibitory activity

SUN Taoli; XU Xiaohui; LU Xinhua

doi:10.3969/j.issn.2095-3097.2016.04.003

Abstract：Objective To compare three extract methods of total flavonoids from Sedum spec-tabile Boreau, and explore its α-glucosidase inhibitory activity. Methods The water, ethanol and ultrasonic extract methods were used to extract total flavonoids from Sedum spectabile Boreau, quali-tative identification used hydrochloric acid-magnesium powder reaction and TLC, the content of total flavonoids was determined by ultraviolet spectrophotometer. The model of 96 microplate high-throughput screening forα-glucosidase inhibitor was established, which was used to determine theα-glucosidase inhibitory activity of total flavonoids from Sedum spectabile Boreau. Results Three total flavonoids from Sedum spectabile Boreau were all showed purple in hydrochloric acid-magnesium powder reaction, and the corresponding location on TLC were the same color of the spots, the spots were separated well and the color was clear.Three contents of total flavonoids from Sedum spectabile Boreau, extracted by water, alcohol and ultrasonic were (2. 80±0. 46)%, (5. 62±0. 56)% and (5. 30±0. 62)% respectively. The contents of total flavonoids extracted by ethanol and ultrasonic were higher than those by water extract method ( P< 0. 05 ) . The α-glucosidase inhibitory rates of three kinds of total flavonoids were (38. 30±1. 54)%, (69. 27±6. 67)% and (61. 23±5. 20)% re-spectively. Compared with total flavonoids extracted by water and ultrasonic, total flavonoid extracted by alcohol showed the better α-glucosidase inhibitory activity ( P< 0. 05) , which was comparable to the positive drug acarbose ( P>0. 05) . Conclusion High content of total flavonoids from Sedum spec-tabile Boreau is extracted by 70% ethanol reflux, and shows potent α-glucosidase inhibitory activity.